The researchers chose a concentration of 0.3 mM IAA as the working concentration for any additional studies instead of 1 mM or 2 mM. The American Journal of Physiology-Endocrinology and Metabolism. ATP from glycolysis is required for normal sodium homeostasis in resting fast-twitch rodent skeletal muscle. No correlation between Na + and K + content and oxidative phosphorylation was found.Īdapted from: Okamoto K, Wang W, Rounds J, Chambers EA, Jacobs DO. Inhibition of oxidative phosphorylation was caused by carbonyl-cyanide m-chlorophenylhydrazone (CCCP), an ionophore that allows protons to move freely through membranes. The researchers also examined the effect disruption of oxidative phosphorylation had on Na + and K + content. (Note: The * indicates p < 0.05 versus control.) The results are displayed in Figure 1.įigure 1 Effects of glycolytic inhibition on intracellular Na + and K + content and lactate production with cellular viability measured by LDH release. Cellular viability was determined by measuring the amount of lactate dehydrogenase (LDH) released, as LDH release is an indicator of cell death. After one hour in the media, the muscle tissues were assayed for intracellular Na + and K + content and lactate production. IAA directly prevents the formation of 1,3-bisphosphoglycerate. Rat skeletal muscle fibers were extracted and incubated in normal media (control), glucose-free media (G(–)), and glucose-free media with various concentrations of the glycolytic inhibitor iodoacetate (IAA). Experiments were conducted to evaluate the effects of glycolytic inhibition on cellular Na + and K + concentrations and lactate production in rat skeletal myocytes. This information would provide a better understanding of myocellular damage that occurs during critical illness. Researchers are interested in whether the energy used for ion transport is derived from glycolysis or oxidative phosphorylation. Because normal intracellular levels of Na + are maintained by the Na +K + ATPase, it is important to understand how metabolic energy production is linked to cation transport. It has been found that failure of cellular energy metabolism is a common symptom in septic patients who do not respond to therapeutics. During septic shock, disruption of Na + homeostasis often occurs and leads to decreased membrane potential and increased intracellular Na +. The myocellular transmembrane Na + gradient is important for proper cellular function. Training Opportunities for Residents and Fellows.ERAS® Tools and Worksheets for Fellowship Applicants.ERAS® Tools and Worksheets for Residency Applicants.MyERAS® Application and Program Signaling for 2023-24.ERAS® 2024 Participating Specialties & Programs. Managing Your Finances During Residency.Interview Resources for Residency Applicants.Researching Residency Programs and Building an Application Strategy.Visiting Student Learning Opportunities™ (VSLO®).Choosing a Specialty with Careers in Medicine®.Managing Your Finances During Medical School.Interview Resources for Medical School Applicants.AAMC PREview™ Professional Readiness Exam.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |